Hola a todos:
Tras la última sesión, las directrices del trabajo ya están expuestas. Como primera premisa ya tenemos el título de nuestro trabajo:
"Single mutations in a unique gene explain the non-mucoid phenotype in a soil bacteria", del que se derivan las siguientes conclusiones:
1. Sinorhizobium meliloti is a soil bacteria which easily change from mucoid to non-mucoid phenotype in laboratory culture.
2. S. meliloti GR4 strain shows more frequently non-mucoid variants than AK21 and RMO17 strains.
3. ExpR gene is a good candidate to analyze the mucoid and non mucoid phenotypes.
4. The single mutations T337 and T565 present in the gene ExpR of the mutants GR4-5.1.1. and GR4-6.3.1 respectively affect deeply the ExpR gene and probably explain the non-mucoid phenotype.
Para todo ello debemos desarrollar el manuscrito. Como tarea pendiente debemos asignar las leyendas a las siguientes figuras:
Figure 1. Sinorhizobium meliloti mucoid and non-mucoid phenotypes.
Figure 2. PCR, DNA electrophoresis...
Figure 3. Electropherograms showing the point mutations found.
Figure 4. Consequences of point mutations on ExpR gene.
Manos a la obra. Además de las personas asignadas deberíamos participar todos en todas dando nuestra opinión.
Hurry up!!!
Dr Francisco Martínez-Abarca
Estación Experimental del Zaidín -CSIC
Department of Microbiology
C/ Profesor Albareda nº 1
18008 Granada
Estación Experimental del Zaidín -CSIC
Department of Microbiology
C/ Profesor Albareda nº 1
18008 Granada
Los que indicamos deberian ser capaces de dejar ya algun comentario en relacion a las figuras.
ResponderEliminarAnimo!!
PACO
Leyenda figurta 1:
ResponderEliminarFigure 1. Sinorhizobium meliloti mucoid and non mucoid phenotypes. Morphology on plates of mucoid (A) and non mucoid (B) S meliloti strains grown in TY media for 48 h at 28 ºC. In C, detail under magnification where single colonies mucoid (black arrows) and non mucoid (red arrows) can be observed.
Leyenda figura 2:
ResponderEliminarFigure 2. DNA Electrophoresis of the PCR amplicons obtained from non mucoid variants of S meliloti GR4. (A) Representation of the Genomic location of ExpR gene. 1 and 2 are the primers used in the PCR reaction. (B) DNA electrophoresis of the ExpR gene (below) and NifH (above) amplicons of the nonmucoid variants described on table 1
Leyenda Figura 3:
ResponderEliminarFigure 3. Electropherograms showing the single mutations detected. DNA electropherograms correspondingtoExpR amplicons of the wildtype (wt) and the two mutants: GR45.1.1 (T337) and GR46.1.1 (T565). In arterisk it is indicated the single change detected.Figure 3. Electropherograms showing the single mutations detected. DNA electropherograms correspondingtoExpR amplicons of the wildtype (wt) and the two mutants: GR45.1.1 (T337) and GR46.1.1 (T565). In arterisk it is indicated the single change detected.
Figure 4. Significance of the single mutations detected on ExpR gene. Representation of the Open Reading frames corresponding to the ExpR gene (246 aa) of the wt(A), T337 mutant (B) and T565 mutant (C); note that a stop codon generates a truncated protein of 112 aa and 138 aa, respectively.
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